Regarding cloning in pet HIS TEV LIC vector

More Niyati Jain's questions See All

What binder would be the best choice to modify glassy carbon electrode with a 2D Material like Graphene or MXene?

I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?

23 July 2024 5,776 2 View

Does Nature Scientific Reports waive open access fee for industry authors?

I came across the Green Building and Sustainable Architecture collection under Nature Scientific Reports some weeks ago. https://www.nature.com/collections/gajghaebce The special issue/collection...

10 July 2024 5,533 1 View

Can I do Parallel Analysis for Principal Axis Factoring Method?

Recently I was suggested to do Parallel Analysis and compare with EV>1 to determine no of factors for my scale. However, the scale I have developed uses Principal Axis Factoring and not PCA. If...

30 June 2024 1,813 2 View

How can I draw curve wavelength vs EQE for fixed current of a LED in silvaco tcad. But tonyplot is showing anode voltage instead of wavelength ?

silvaco-tcad

25 May 2024 9,599 0 View

How to extract collagen from bones specimen?

how to extract collagen from bones specimen?

27 April 2024 6,307 0 View

Is there any method to do TDM for TB drug Ethambutol in human plasma by UHPLC with PDA detector?

We are standardizing chromatographic conditions for first-line TB drugs in our newly procured UHPLC system. For Ethambutol, we are not able to get dedicated chromatogram. Can anyone suggest any...

24 April 2024 9,859 0 View

Recently we are develping methods for Therapeutic drug monitoring of TB drugs, Need to know the required calculation for final estimation of drugs?

Hello, everyone. This is Dr Prerna, SR Biochemistry, NITRD, New Delhi. We are developing methods for Therapeutic drug monitoring of first line TB drugs in our laboratory. After utilizing suitable...

17 April 2024 9,598 0 View

What is Quantum Computing?

With Key Concepts.

04 April 2024 538 3 View

Can someone provide C33A cell line in India?

I am working on cervical cancer. I have cervical cancer cell line, but for negative control non cancerous cervical cell line is not available with NCCS, Pune. So can someone provide me C33A cell...

01 April 2024 1,357 2 View

What are various properties of Search Algorithms in AI?

Explain in Detail.

24 March 2024 8,833 3 View

I did hirshfeld surface analysis for molecule but did not find red spot and interaction surface in energy frame work, is it ok for research article ?

In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?

28 July 2024 2,988 0 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

Using a large amount of data, such as 30 years of daily data, in a machine learning model like LSTM can reduce the model predictivity?

I have used 30 years of daily data, but the model is not able to optimize, getting just 0.57 in NSE and R2. when I reduced the data to 20 years in daily time frame I got 0.73 in NSE, R2 for both...

22 July 2024 669 0 View

Recommendations for his-tag magnetic beads / resins?

Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...

16 July 2024 3,692 6 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

What should I keep in mind when designing a gene construct when trying to co-transform to increase protein expression?

I am having trouble expressing a protein and have heard that co-transforming it can improve stability and increase expression, so I am looking to try this experiment. I only need the kinase domain...

08 July 2024 2,935 5 View

Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

Regarding first author name in articles ?

Dear Sir, Four publications in my profile do not belongs to me belongs to MALLAMMA, University of Mysore , Mysore: They are 1. Kinetic and Mechanistics studies of Pd(II)...

29 June 2024 1,732 0 View

Why does my protein bind poorly to the Ni sepharose beads?

I am purifying RNAse E in E. coli (118 kDa). I cloned the rne coding sequence on pET28a with a His tag at the N-terminus. The protein overexpressed fine (see attached figures). The binding buffer...

24 June 2024 6,287 4 View