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Has anybody seen the jiggling of FPLC trace like this? I have cleaned the flow cell which was hinted to be the cause of this with 0.5 M NaOH followed by 500 ml wash with MilliQ water. Still, I am...
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I am trying to clone one of the construct which don't start with Methionine do I need to add ATG in my primer or I don't need it does ATG site is already in frame in this vector starting before...
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