How to properly calculate the mutational rate in bacteria?

Hello everyone, I hope y'all can answer a simple but problematic question. I have been doing mutation accumulation experiment in 3 parallel lineages in bacteria and I now want to calculate the...

05 December 2019 5,648 4 View

Is anybody know an effective antibody to detect mScarlet-I signals?

Hello everyone! I would like to visualize the expression of my protein with western blot using plant cell-lysate. My plant contains my protein tagged with mScarlet-I. First, I wonder if I can use...

09 July 2019 6,203 2 View

Does anyone having some suggestion to improve Arabidopsis protoplasts transfection using the Tape-sandwich method?

Hello everyone! I am a student and I am trying to express fluorescently tagged proteins in Arabidopsis using the Tape-Arabidopsis Sandwich method. I isolated and got nice intact protoplasts....

27 April 2019 5,845 3 View

How to separate two organic salts?

I would like to know how can I separate two organic salts which I have in the same sample. I want to purificate both and characterize but I dont know how could I do it Another question is how to...

23 October 2018 4,429 3 View

Has anyone performed QIIME analysis using Illumina (MiSeq) demultiplexed data?

14 November 2016 6,362 4 View

What program can I use to make a in silico PCR and throw me as result an agarose gel with my expected fragment?

What program can I use to make a in silico PCR and throw me as result an agarose gel with my expected fragment?. Only putting my sequence of interest, my primers and the expected size.

12 September 2016 3,379 1 View

How can I coat an IrO2 on Ti substrate electrode with liquid Nafion dispersion?

Hi all, Currently I'm trying to coat the IrO2 electrode, which acts as anode, with a liquid Nafion Dispersion using a dipper. However, this coating does not work as chlorine is produced after...

28 March 2016 174 8 View

Physical activity and telomere shortening

What is the current evidence regarding the relationship between telomere shortening and exercise??

01 January 1970 7,894 4 View

Physical activity and telomere shortening

What is the current evidence regarding the relationship between telomere shortening and exercise??

01 January 1970 275 7 View

Exercise is an overrated method for weight loss??

Exercise benefits for health are well known, but what if we're looking for the best way to fat loss, it's really exercise as important as most people think or is kinda overrated??

01 January 1970 7,431 58 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View