I have received data from mass-spec about the possible interactors finding for a protein. The problem is that now I have a list of multiples interactors and obviously I can not try to verify all of them by Co-IP or PLA.
For that, I would like to know if is there any technique/protocol/website for trying to reduce a list of interactors and choose the most possible, trying to avoid the the "worst" candidates?
Hi Yeray Hernández-Reyes
You can take the entire list of proteins obtained from mass spec and use the STRING's website (https://string-db.org/). In the settings section, you can edit the minimum required interaction score: highest confidence (0.900). You can also edit max number of interactors to show: no more than 5 or 10 and UPDATE the analysis. Again under active interaction sources: choose Experiments or whichever is convenient to you.
Hope this helps!
Varsha
I'd also suggest you take a look at this; https://www.crapome.org/
Depending on your expertise and what data you have access to, you might also want to look at this; http://saint-apms.sourceforge.net/Main.html
https://www.nature.com/articles/nmeth.1541
- Badges
- Science topic