What causes "bubbles" in DNA gel electrophoresis bands?

Hi all, I just ran a gel electrophoresis that shows a bubble shape in the bands of the ladder. No other row had this shape, and I haven't seen it before. What causes this? Is there anything that...

02 December 2020 1,380 4 View

How to calculate the amount of glucose from the color of Benedict solution?

I'm planning to use Benidict solution to do research on the amount of glucose in candy, but I'm not sure how to calculate the specific amount of sugar from the Benedict solution. Is there a way to...

10 November 2020 1,380 3 View

How to conduct moderation analysis using PROCESS in SPSS with 2 categorical IVs, 1 continuous moderator and 1 continuous DV?

I'm using Hayes' PROCESS macro in SPSS to carry out a moderation analysis. I have two dummy variables (IV1 and IV2), one moderator variable (W) and one DV (Y). As the dialog box does not allow...

10 March 2020 6,652 3 View

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

21 November 2019 408 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

Acidification of biochar to lower its pH?

I am trying to test my biochars (charred biomass residues) in ammonia absorption tests. The more acidic biochars (hydrochars) work better, they have a lower pH than the high temperature chars....

11 October 2018 181 7 View

What could be the cause of the double bands showing after running my PCR product on a gel?

After running my PCR on a gel, I got double bands showing for all the positive samples. However, the expected band was brighter than the other band I observed. The cycling conditions were 94 C- 5...

26 March 2018 1,676 3 View

What is wrong with my Western Blot detection?

06 June 2017 4,792 4 View

What software is now being used in place of KC Junior for BioTek platereaders

We used to use KC Junior to run our Elisas and XTT assays as well as our luminometer. Having installed new computers on our plate readers we now are informed that KC Junior is no longer available...

14 April 2016 1,190 3 View

If two variables covary, how do you interpret it?

18 February 2016 9,537 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View