Can someone please share how to add a spherical wrap / correction to a visual stimulus presented to mice using python?

Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...

22 November 2020 7,905 2 View

Does my laptop (AMD Ryzen 4300G with AMD Radeon Graphics) suitable for YASARA MD simulation?

My current laptop is Lenovo Flex 5, AMD Ryzen 4300G with AMD Radeon Graphics. I have just researched that most people recommended that Nvidia series of graphic cards but my budget can't allow me...

25 October 2020 4,777 3 View

How do I find out what publications cited my article?

How do I find out what publications cited my article? Ronald Quinn

11 October 2020 762 4 View

How to reduce background of Fluo-4 in flow cytometry of epithelial cells?

I'm trying to measure the calcium increase in epithelial cells (fresh, isolated from mice) after receptor activation using flow cytometry. This is the protocol I follow: after isolating the cells...

29 June 2020 2,231 3 View

Best GST antibody for Western blot?

Hi, I am performing GST purification, however, I am getting poor signal with my GST antibody at the recommended dilutions -...

11 June 2020 9,769 1 View

Can anyone tell me the largest number of proteins directly extracted from soil ?

Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...

30 January 2020 9,060 2 View

I am doing my grad dip in critical care and I am struggling to find a research topic?

My topics and questions are only in the early stages and haphazard. It will be a literature review. So far I am thinking of the effects of sedation breaks in adult ICU patients. Early...

21 February 2019 2,819 9 View

We are looking for a Candicidin D standard ?

We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?

15 January 2019 2,482 3 View

Are there any software pipelines for processing HCID CID data in TMT experiment ?

I performed some comparitive proteomics between planktonic and biofilm membrane proteomes using tandem mass tags. The mass spectrometry generated HCID and CID data . I now need to identify all...

13 August 2018 5,971 3 View

Are there specialised methods for extracting DNA from alkaline soils ?

I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.

03 August 2018 2,101 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View