I'm looking to the best way to approach Receptor Occupancy (RO) determination in peripheral tissue for a monoclonal antibody targeting a cell surface protein in a peripheral tissue. For many monoclonal antibodies it's a common practice to study RO in blood cells by Flow Cytometry, if mAb is targeted to a surface proteins of blood cells (Lymphocytes, etc.), but about RO in non-blood tissues, I have not found any clear approach, I mean peripheral tissues like colon, pancreas, liver, etc..
Is it possible, in your experience, to measure the RO in peripheral tissues by direct detection of bound antibody?
Are there preferred approaches, techniques, etc.?
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