Does anyone know if it is more realistic to use Ct, amount of DNA (in pg DNA) or number of gene copies in Real-time PCR assay? I used number of gene copies before in 2006 but very few research articles seem to use this now. Why? if we use the standard curve equation, it is likely to deduce the number of gene copies as few as tens to thousands of copies. Does this meaningful? Some journal uses detection range as 0.4 pg, which is 100000 gene copies. Is it enough for pathogen detection?
Regards,
Alvin
For pathogen detection by Real Time PCR generally template DNA is isolated from infected or symptomatic part , Hence it is more relevant to mention the amount of DNA used for detection and then correlate it with copy number. Even the information about the range of Ct value for positive samples may also help you to interpret correct results. I had used this approach for detection of pathogen in citrus.
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