Hi!!
I am looking to use the RAW264.7 cell line for phagocytosis assay. Does these cells naturally exhibit phagocytosis activity?
Or is it necessary to activate these with LPS (as I read from articles)?
If it needs to be activated, could you please share the protocol.
Thank you,
Check out https://www.researchgate.net/topic/RAW-2647. To my knowledge, these immortalized mouse macrophage are naturally phagocytic but can also be induced by treatment with LPS.
yes, they're phagocytic. (https://academic.oup.com/biomethods/article/2/1/bpx002/3064667) and highly responsive to pro-inflammatory stimuli.
Ben Stevens Thank you.
I thought of using the pHrodo red E.coli particles (https://www.thermofisher.com/order/catalog/product/P35361) for phagocytosis assay using RAW264.7.
May I perform the assay without any external stimuli such as LPS. Does it work?
Hi Rathina, I was working on the same problem and may I know the stimuli is necessary for studying phagocytosis of RAW264.7?
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