Rathina Kumar Shanmugakani

6 Questions 12 Answers 0 Followers

Questions related from Rathina Kumar Shanmugakani

How to isolate gram positive bacterial cell membrane proteins (both outer and inner) and proteomic analysis?

I wanna compare the cell membrane proteins in two different cell types of the same bacteria. They are not overexpressed by any expression systems. The two cell types just belong to two different...

21 July 2021 2,722 5 View

How to confirm whether a particular compound is having killing effect or reducing the growth rate of bacteria?

Hi, I am studying the effect of a particular compound on a bacterial pathogen. By treating the compound with the bacterial culture, I found that the CFU is reducing. Then, I checked the growth...

11 May 2021 4,946 1 View

Isolation of macrophages/monocytes and neutrophils?

I am looking for a protocol to isolate the phagocytes (macrophages and neutrophils) from whole blood. Could you please give me your sugeestion on an efficient method. Thank you,

12 August 2019 8,501 5 View

Temperature for PMCs isolation?

At what temperature, the centrifugation should be done to isolate PBMCs from whole blood using Ficol gradient centrifugation? I found different protocols saying different temperatures during...

03 June 2019 4,160 6 View

RAW264.7 for phagocytosis?

Hi!! I am looking to use the RAW264.7 cell line for phagocytosis assay. Does these cells naturally exhibit phagocytosis activity? Or is it necessary to activate these with LPS (as I read from...

12 February 2019 592 5 View

Any suggestions on the one non-specific band in SDS PAGE?

Dear all!!! I am purifying a protein of 30 kDa MW. I purify through Ni-NTA agarose - anion exchange chromatography and finally with size exclusion chromatography. After the final purification, I...

18 April 2015 4,415 3 View