I recommend you try HumImmu products for Rapid Test for coronavirus disease (COVID-19).

Coronavirus (2019-nCoV, SARS-CoV-2) IgM antibody rapid diagnostic test

http://humimmu.com/coronavirus-2019-ncov-sars-cov-2-igm-antibody-rapid-diagnostic-test.html

Coronavirus (2019-nCoV, SARS-CoV-2) IgG antibody rapid diagnostic test

http://humimmu.com/coronavirus-2019-ncov-sars-cov-2-igg-antibody-rapid-diagnostic-test.html

Thanks Kevin Lee,

That was helpful. Developing a POC product in such a short time is really surprising.

PCR IS RAPID TEST FOR INVESTIGATION IT TAKE ONLY FEW HOURS AS I KNOW .

Anyone knows if any lateral flow test has been developed for the direct detection of the viral particle in human fluids?

I was impressed by the velocity of that development, however these companies probably already had a basic design for testing mucus, blood or saliva. Changing the type of IgG they detect is not too difficult. With a bit of luck you could get an ok test fast

I was also impressed by such fast development. Just by simply changing the IgG type, almost all of the preparation steps will be changed from the conjugation to immobilization and stabilization, and .... . The optimization of lateral flow test strips is a tedious and time-consuming procedure. However, as said by Héctor Osorio-Vega , having a previous knowledge may facilitate the test development rate.

Real-Time RT-PCR 

Please correct me if I am wrong, but I think that the current tests only detect antibodies for the virus and not the actual virus directly, anyone has information about that?

That's right. The rapid test is based on the detection of the corresponding IgM antibody.

The RT-PCR is not a rapid test.

Hey, good question: check out my blue paper on how it can be done with greater specificity and accuracy using somewhat novel approaches:

Preprint Covid-19 Rapid Test - BLUE PAPER

Moreover, there are a few companies that have working test already (as per online info):

1:

https://www.biomedomics.com/products/infectious-disease/covid-19-rt/

2:

https://www.biopanda.co.uk/php/products/rapid/infectious_diseases/covid19.php

All the best!

Thanks #Mikael Franzen for sharing the file. I am familiar with the design of a rapid test. I am just looking for an institute or company which can support the financial costs for the development of such a test.

Tohid Mahmoudi - Good for you. There are different ways to design stuff, right, and some ways may work better than others....so I'm just sharing with you...take it or leave it; it's up to you. So, I'm also interested in the financial support part of the question and as far as I'm aware there is no serious effort that has been communicated. At least not here in Sweden.

The current method using for testing samples from infected patients is qPCR (quntatative PCR, not conventional PCR). At least the test kits sent out to states by CDC in USA is based on qPCR. It comes with a protocol for the state hospitals/clinics to follow and test. All are documented and can be found in CDC (Centers of Disease and Prevention) website.

There is a good discussion on RG about "Why is the Coronavirus SARS-CoV-2 detected by qPCR?" See below link:

https://www.researchgate.net/post/Why_is_the_Coronavirus_SARS-CoV-2_detected_by_qPCR

Yuan-Yeu Yau : qPCR makes sense. Still, I think we need a quicker method for POC-use; MDs all over the world are asking for it as they can't order a standard test for everyone who has a bad cold.

A piece of info for qPCR detection. The production of such testing kits is quickly scaling up here in the US. The number of produced kidt s is reaching 5 million in only a few weeks. Reported by GEN (Mar. 12, 2020). In the begining , the testing capacity in the US was not enough and the test design was confusion. But, now I can see it is on the right track and in its full power. I like IDT, I used to use its site for designing my oligo mers. See part of this article below:

----------------------------------------

"SARS-CoV-2 Detection Kits Primed by IDT’s Quick Scale-Up"

For Trey Martin, the president of Integrated DNA Technologies (IDT), the last few weeks have been anything but typical at the Coralville, Iowa (USA) company. Indeed, IDT finds itself in the center of the COVID-19 outbreak response. When the outbreak reached the United States in February, the CDC and FDA reached out to the well-known maker of DNA oligos to provide a key component of SARS-CoV-2 detection kit production—the primers that recognize the viral genome. Martin says that IDT gladly accepted.

IDT makes the primer probe kit, just one of four parts of the detection kit workflow, which is used alongside the RNA extraction kit, master mix, and thermocycler. Each kit, or 2019-nCoV CDC qPCR Probe Assay, contains 4 qPCR amplicons including an RPP30 control—all developed by the CDC. Available for order on the IDT website, the kit provides enough oligos to perform 500 samples and costs $125, making each reaction cost a quarter (25 cents).

The kit contains primers and probes that have passed functional testing at the CDC and may be used with the CDC 2019-nCoV Real-Time RT-PCR diagnostic panel instructions for use under CDC’s Emergency Use Authorization (EUA). The primers currently target the N1, N2, and RP genes of the virus, but these are subject to change. For more information on the RT-PCR panel, please see the FDA website.

To read more:

https://www.genengnews.com/news/sars-cov-2-detection-kits-primed-by-idts-quick-scale-up/?utm_medium=newsletter&utm_source=GEN+Daily+News+Highlights&utm_content=01&utm_campaign=GEN+Daily+News+Highlights_20200313&oly_enc_id=8464I0071034B8S

Special thanks to Dear Dr. Yuan Yu and Dear Dr. Yuan-Yeu Yau for useful comments. And I hope to see POC devices for the fast detection of this disease too.

Novel Corona virus (COVID 19) has gripped the world. This life threatening viral disease can be diagnosed by employing quantitative PCR (qPCR) technique. In addition, real-time RT-PCR assay is also developed for testing clinical samples from patients for COVID-19 infection.

Serologic IgM and IgG rapid detection:

https://www.confirmbiosciences.com/coronavirus-test/

Rapid molecular Covid 19 detection:

http://cepheid.mediaroom.com/2020-03-21-Cepheid-Receives-Emergency-Use-Authorization-from-FDA-for-Rapid-SARS-CoV-2-Test

https://www.researchgate.net/post/Is_there_any_specific_biomarker_that_could_be_used_for_rapid_testing_of_COVID-19

Usually, RT-PCR or qPCR based test assay is performed for the diagnostic tests, which seems a little bit time-consuming. On the other hand, some rapid tests based on IgG-IgM interaction have been mentioned, which probably is not a specific test for coronavirus COVID-19. That is why, finding specific biomarkers (like antibody, protein or others) could be a very useful means to produce effective testing kits especially microdevices like lateral flow immunoassay strip or electrochemical sensor (lab on a chip). Can anybody, please, give me specific information or reference regarding this issue?

As mentioned by Antonio Alonso , these two quick methods are now been authorized to be used in the US for conoravirus detection to expand testing capacity. Turnaround time using IgG/IgM is about 10-15 minutes. Turnaround time for using Cepheid's product is around 45 minutes. IgG/IgM detection needs blood sample. Cepheid's method does not need blood sample. Instead, samples can be simply taken from mouth.

Outcome of emergency funding action for coronavirus SARS-CoV-2 outbreak: 17 new projects.

Hi Tohid Mahmoudi

You may find the following links useful:

https://www.nature.com/articles/d41587-020-00010-2

https://www.finddx.org/covid-19/pipeline/

https://www.milenia-biotec.com/en/is-it-possible-to-develop-a-covid-19-test-via-the-lateral-flow-test-milenia-hybridetect/

https://techcrunch.com/2020/03/25/uk-researchers-develop-new-low-cost-rapid-covid-19-test-that-could-even-be-used-at-home/amp/

سلام جناب محمودی .

آیا تجربه یا دانش فنی در حوزه تولید کیت های لترال فلو دارید؟

در این خصوص بیشتر توضیح دهید - در خصوص سرمایه گذاری برای تولید شرکت را میشناسم که آماده است

پیروز باشید

مختاری

[email protected]

What if biomarkers used for RT PCR or qPCR were used in this device mentioned in the next article as POC?

https://www.laserfocusworld.com/test-measurement/article/14173589/new-saliva-test-to-instantly-detect-coronavirus-via-interferometric-laser-technology ?

Thanks for all responses and comments and I hope the researchers will find soon a rapid test for COVID-19.

Dear Daniel Grajales ,

The aforementioned device (CONVAT project, ICN2; Barcelona, Spain ) is a label-free, optical, microfluidic instrument for the rapid detection of antibody-related to the COVID-19. Among the various bioreceptors, antibodies show better performance from different aspects.

LFIAs benefit from less cost than any other rapid tests and it is more desired. However, the COVAT device is unique in its kind.

Thanks for sharing it.

This is an interesting topic, and an area of competitive research. So far there is not a confirmed agreed rapid test yet. And studies evaluated some tests are not reliable enough for such purposes. [1] Testing Individuals for Coronavirus Disease 2019 (COVID-19).

JAMA.

doi:10.1001/jama.2020.5388 [2] Performance of VivaDiag COVID‐19 IgM/IgG Rapid Test is inadequate for diagnosis of COVID‐19 in acute patients referring to emergency room department.

J Med Virol.

doi:10.1002/jmv.25800

University of Insubria Varese Italy is working on a rapit salivary.

The researcher is Lorenzo Azzi.

Any rapid test kit developed in Africa?

I am glad to say that currently there are many providers of LFIA rapid tests for SARS-CoV-2 (COVID-19).

Thanks for all of the researchers.

Yes ! Dyne Immune Institute is currently developing firstly, a rapid, non-instrument based hand held, 15 minute assay for detection of the COVID-19 virus in saliva,

sputum or serum..."ONSITE". Concurrently, we are also developing a high throughput automated assay, again, for the virus...not the subject antibody response. The assays will detect virus present in subjects who are asymtomatic, but infectious.

Dr. Mike Huchital,

Chairman & Chief Scientific Officer, DYNE IMMUNE INSTITUTE.

Please have a look at the following attached document that discusses the rapid tests for COVID-19.

#REASSURED criteria

In india the Standard Q COVID-19 rapid antigen test has been validated and has been put to great use in the entire system.