I'm running gel for electrophoresis process. At first, there are bubble after I switch on the machine. Then, I let it for about 1 hour. When I check it after that, there are no bubbles and the machine isn't warm at all. I don't know what happen with it. Then, I realise the white wire in the red side inside of the machine is not in place as in the black side. Could it be the reason? Or is it about the buffer? Really hope someone can help me. Thank you. Below is the image of the white wire.
Hi
The problem indicates that electric current is not flowing between the electrodes. Carefully see what you power pack is indicating. If it is showing set voltage but no current when you run the gel, it indicates the 'circut is not closed'. This can be due to the electrode or some problem in the power pack. Connect it with another gel apparatus and see it that works..if not get in touch with a technician
Hi
The problem indicates that electric current is not flowing between the electrodes. Carefully see what you power pack is indicating. If it is showing set voltage but no current when you run the gel, it indicates the 'circut is not closed'. This can be due to the electrode or some problem in the power pack. Connect it with another gel apparatus and see it that works..if not get in touch with a technician
It is likely that one of the electrode wires is broken This usually happens where it is held in place by the banana connector which screws into the acrylic case. Possibly the current flows either because the connector is wet with buffer or the wire is just touching the copper connector then the wire heats up and become disconnected so no current flows. Sometimes these soft platinum wires break if the gel apparatus is too vigorously cleaned. If you disconnect everything then touch the wires gently they should not move. If the wire moves then it is probably broken and will need to be removed and re connected into the banana connector either by your lab manager or the company supplying the gel tank
Hi,
You may check for the following:
1. There can be deposition of salts/dirt or corrosion of the electrode wire. Try cleaning gently. Since bubbles are appearing in the beginning, the wire doesn't seem to be broken. Still, it needs a check.
2. Also check the connectors carefully for depositions.
3. After the bubbles appear have a look at the voltage and current.
4. Using fresh buffer, run a gel, and see if it is affecting the run. Due to the temperature of the room, the apparatus doesn't heat sometimes.
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