There are many factors in environmental systems that can interfere with the fluorescent properties of fluorescent substances, such as metal ions. Is it possible to design a fluorescent reagent whose fluorescence performance is not interfered by any ions?
Dear Jiaqi Chen Not sure what your point is but in general fluorescent probes are insensitive to metal ions (at high concentrations ionic strength sometimes might be).
As a matter of fact there are fluorescent probes specifically designed to monitor (certain) metal ions:
https://www.thermofisher.com/nl/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/fluorescent-indicators-for-zn2-and-other-metal-ions.html
in rare cases some metal ions quench fluorescence (of Trp fluorescence for example).
Best regards.
Rob Keller Thank you for your answer, perhaps I was rather unclear in my question. I was wondering if there is a way to make the fluorescent agent more stable and resistant to interference, and its fluorescent intensity is almost unaffected by external factors. Beacuse, the fluorescence was used in the water system to achieve the fluorescent tracer detection, but there are some ion in the system, like the iron ion, and its concentration is 2 mg/L. In most fluorophores, such concentrations of iron ions cause changes in fluorescence intensity that are detrimental to fluorescence tracer detection in water systems, because of the change in fluorescence intensity is not only due to the fluorophore concentration in the above condition.
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