Signal phase shift after analytical calculation?

Suppose we have a signal as x(t) = exp(i(omega*t + phi)), after a simple calculation as y(t) = 1/x(t). We can clearly see that there is a phase shift of Pi=180deg. However, using directly...

20 October 2020 4,923 5 View

Is it necessary to use ethanol to drain off glutaraldehyde?

Hi everyone! I'm using glutaraldehyde as a spacer to cross-link a primary amino and an enzyme on plat glass. After the step of immerging the support in 2.5% glutaraldehyde solution, one protocol...

20 July 2020 3,702 2 View

ORCA4.2.0 GTOInt error?

when I run ORCA, I got an error as follows: ORCA finished by error termination in GTOInt Calling Command: mpiexec -np 4 D:\ORCA421\orca_gtoint_mpi.exe na20.int.tmp na20 [file...

08 May 2020 5,444 3 View

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As you can see in the picture, the salt is stuck to the crucible. Anyone has experience of melting salt at high temperature? Would stainless steel crucible be better?

04 March 2020 3,412 3 View

Why should SVD get two equal singular values for a single mode?

Singular Value Decomposition (SVD) is an important method in the area of signal processing. I try to use SVD to calculate the MHD mode structure from experiment data. However, during the benchmark...

10 April 2019 6,118 4 View

How to derive the Boltzmann equation in plasma physics?

Boltzmann equation in plasma physics is a very fundamental equation in kinetic theory. What's the relation of the one in plasma physics and that in thermodynamics.

03 April 2019 4,925 8 View

How to justify Increase in activation energy by increasing GDC/SDC?

how can we just justify the increase in activation energy by increasing the GDC/SDC content in sofc cathode..... for reference please see this paper page 6/2165.... DOI...

03 March 2019 3,132 3 View

C.elegans plate: what is the reason for the agar concentration of 1.7%? Can I increase it?

I am doing lifespan assays for ~35 genes simultaneously in C. elegans on FUDR (inhibiting reproduction) plates to avoid re-picking the worms. I tried it once and got positive results, but a...

06 February 2019 728 4 View

How about the minimum quantity/concentration/volume that I should add into wells when Gel electrophoresis?

In DNA/RNA Gel electrophoresis, which is the key factor, total quantity, or concentration, or volume, that may influence the lightness of bands?

05 August 2018 9,377 8 View

How the overall score is related to their subscale scores?

This is the third section of Items Decomposition: How to structure the Scores and Items. This section describes, using the graphical representation, how the score associated with all items in a...

07 June 2018 8,942 4 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

How does trust influence the development of environmental projects?

Hello Scholars, I am exploring "trust" relevance in the development of socio-technical systems. What is your perception of cognitive values like trust to the energy transition/implementation of...

09 February 2021 8,133 5 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Where can I find the information about steel final production in Japan?

I am doing research about the in-use stock of iron and steel in Japan. I need the report about annual yield of steel final production. I found some information in the webpage of Japan Iron and...

25 January 2021 3,867 2 View