I am pursuing a project that will require me to quantify excitatory synapses in rat spinal cord slices (fixed in PFA). I have a number of references and protocols which describe methods for quantification, but they all use tissue that is 5-15 uM thick.
I would like to use 20 uM thick slices (for a number of other standardized outcome measures), but am concerned that this could be too thick and create too much background/reduce resolution. If you have experience quantifying synapses in fixed tissue, do you believe that this would be an issue?
Hi Khalid,
Look at this paper: 10.1007/s00429-016-1278-x ( i have helped for the quantif and it was ok, as long as you do the deconvolution step!). have also a look to this one: 10.3389/fncir.2013.00153
Hope that helps!
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