Yes. See

M. Imgratta, J. Hollinger, J. Duhamal, J. Am. Chem. Soc., 2008,130, 9420.

Amount of fluorescence will depend upon whether in solution or solid.

There can be different reasons. Before going any further.

How was the labelling done and the polymer purified?

Have you removed free pyrene i.e. pyrene which is not attached to the polymer?

It also depends on the excitation wavelength. What wavelength are you using for excitation?

How are doing your experiment? Are you studying the sample in solution phase or solid phase? This is important because fluorescent quantum yields differ in solution phase and solid phase.

Unless I have all these answers, it will be difficult for me to provide you with an exact solution for your problem.

Thank you for your answers,

I will try to clarify it a little bite more, we are grafting pyrene on monolithic polymer (used in capillary for chromatography ) the analysis was only made in solid phase. The wavelength used was 364 nm. It was purified by passing solvent through the column for certain amount of time (normally we should remove most part of physisorbed pyrene). We think we are grafting the pyrene on the monolith by a radical process (but not 100% sure).

again thank you I hope you will be able to help me with these clarifications.

I made a mistake the laser wavelength was 638nm.

632.8 nm ？？？？？

it is 633 nm for

Renishaw inVia Raman system！！！！