What is the best treatment to block autophagy in C2C12 cell line?

I read that NH4CL and chloroquine treatments can be used to block autophagic flux. Are there any other acceptable treatments?

02 September 2020 4,252 3 View

How to generate heterozygous knockouts using CRISPR Cas9 in iPSCs?

Hi all. I'm trying to create a heterozygous deletion (55bp) mutation in iPSCs to model malignant tranformation. I'm using two sgRNAs in a plasmid based system (puromycin selection). I obtained ~20...

18 May 2020 5,389 1 View

Are there guidelines for conducting a meta analysis and scoping review on case reports?

Currently, I can only find case reports on the rare condition I am planning to conduct a meta-analysis on. I will be evaluating outcomes and efficacy of treatments. Are there any known guidelines...

24 February 2020 5,742 3 View

5x5 crosstabs table on spss, iteration keeps going up and no results , anyone know why?

Hey all, So im trying to run 5X5 crosstabs with fisher exact test on SPSS, got about 1000~ lines in the file. Im seeing the iteration number keeps going up (1700 currently), but nothing happens...

07 December 2018 5,602 4 View

How to oxidize Nitric oxide?

20 June 2016 2,008 9 View

Where can I purchase Silica nanoparticles?

In the past I worked with silica nanoparticles bought from "Nissan Chemicals", Product No. ST-40, see link for other types.In the company site I can't find that silica anymore.How can I get that...

17 August 2014 6,289 10 View

What are the best ADHD diagnostic methods, given that my goal is to associate between risky driving and sub-impairments (or sub-dimensions) of ADHD?

Hi, I am studying the links between ADHD sub-dimensions and the different types of risky driving. What are the best ADHD diagnostic methods, given that my goal is to associate between risky...

01 January 1970 2,805 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How can I add a new WSN routing protocol in NS2 or NS3?

I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...

19 February 2021 7,981 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

How can I calculate teh crystallinity degree of graphene oxide?

I had treat my XRD diffraction pattern by Highscore to calculate the crystallinity degree Xc and I have the formula but I dont no the 2Theta of Ac and Aa to calculate Xc. Is it like polymers...

10 February 2021 6,305 5 View

Could any one help me with mechanical properties of CFRP(carbon fiber reinforced polymers)?

I am modeling CFRP laminated a concrete column in Abaqus And I need Elastic and plastic properties of CFRP

09 February 2021 4,930 5 View