Puromycin kill curve for CHO-S (suspension) cells

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Sudip Dey

check this article

Article Retrovectors packaged in CHO cells to generate GLP-1-Fc stab...

Sarmishta Majumdar

Thanks Sudip Dey Are you aware of the protocol to study the kill curve?

I didn't use for CHO cell line but I have done stable transfection and Puromycin was used for selection and cells are dying. Sarmishta Majumdar

At what conc.? And which cell line did you use if you can let me know

U20S cell line with 1ug/ml. You can go for MTT or take the above mentioned paper as reference as they have used CHO-S cell for stable transfection.

Sudip Dey I'll try.. Is MTT assay necessary or usual viability study over a period of 10 days should be enough?