Role of passive components in RF transceiver system design?

I am unable to quantify the role of passive components when it is attached with the antenna. For various frequency bands like L,S,C,X the specifications of the active circuits changes but what...

25 August 2020 1,463 4 View

Question on detection of DNA Protein covalent complexes by dot blot. How to achieve reproducubility?

I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...

30 July 2020 949 4 View

What are the artificial intelligence models which can apply to estimate river bank erosion potentiality?

I am working with such kind of models

20 July 2020 7,496 4 View

What are some good reading materials for a course on Introduction to Qualitative Research?

I am designing an outline for an introductory course on Qualitative Research for PhD students, and looking for research papers (conceptual/empirical) that may be recommended to the scholars.

28 May 2019 2,736 5 View

Is there a in-built function in MATLAB for identifying an arbitrary point with a particular voronoi cell?

I have a voronoi cell structure. I also have some arbitrary points. I need to know if there is an in built function in MATLAB to identify which arbitrary point comes under which point's domain of...

09 April 2019 8,770 1 View

What are basic differences between data mining and machine learning?

Can anyone clearly explain the differences between data mining and machine learning and whether a classifier comes under data mining or machine learning (e.g. MLP, SVM etc.)?

31 March 2019 5,888 9 View

Identifying PGE by SEM and XRD. Will it be possible to get more or less same results by the Fire Assay method?

Getting result of SEM and XRD with full positivity in PGE concentration in a gabbro-anorthositic layered complex but not getting full support when analyzed by fire assay method.

14 February 2019 423 3 View

Why do metal nanoparticles produce metal ions in aqueous solutions?

I am working with copper nanoparticles. These nanoparticles are producing Cu2+ ions in PBS. I want to know that whether these ions release is somehow related to the loss of conduction electrons...

06 June 2018 4,977 4 View

Oil is spread on glass and a drop of water is put on it, how to visualize (3D) it using SE FIT or any other software?

Soft condensed matter, SE FIT

31 July 2016 3,102 3 View

Is it possible to do statistical data analysis by Mahalonobis method?

My data comprises of bulk geochemical i.e.,major, trace and REE parameters of granitoids. Mineralogical and norm calculation do not show much variation of data. However, I can correlate data...

13 June 2016 8,414 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

Problems with library virus generation?

Dear all I cloned an sgRNA library into a pLenti plasmid backbone (puromycin selection marker) by golden gate ligation and transformation. However I cannot produce any virus from the library...

25 October 2020 3,820 2 View

Antibiotic screening of drug resistance cancer cell line with MDR pumps?

Hi. during our study, we faced with a problem in the screening of EPG RDB 257 cell line which is a resistant cell line due to the expression of efflux pumps such as MDR 1 which actively excrete...

27 August 2020 9,192 1 View

Post transfection contamination ?

i am trying to transfect suspension cell line for that i use lipofectamine 3000 along with reduced serum media without antibiotics (optimem media) 6hr of duration for transfection .After 48 hr i...

19 August 2020 894 3 View

What selection markers to use for the generation of stable HEK293 cell line ?

Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...

14 August 2020 7,542 2 View

What puromycin concentration would you use for selection in WM3211 melanoma cell line ?

24 May 2020 2,728 4 View

How to create Stble cell line for membrane proteins?

Hi I want to create stable cell lines for membrane proteins of interest in inducible expression system. I wonder if anyone has used pTRE-tight-BI system to express two genes simultaneously in...

12 March 2020 2,832 3 View

What is wrong with discrepancy of sequencing results between gDNA and cDNA after CRISPR/Cas9 gene editing?

Hi everyone, I am trying to use CRISPR/Cas9 to mutate one gene in leukemia cell lines by Lipo3000 (from AGC to AAC). After transfection and puromycin selection of 5 days, the GFP+ of the cells is...

31 December 2019 7,006 3 View