Paul Taslimi

5 Questions 6 Answers 0 Followers

Questions related from Paul Taslimi

Purification of Apolipoproteins from plasma?

Anyone have experience with this? I am trying Dextran sulfate/manganese to get at LDL and VLDL first. But the literature out there is confusing. Would appreciate some advice on the protocol if...

03 April 2024 5,181 1 View

Maintenance and culture of iPSC derived Macrophages?

I was wondering if anyone out there works with macrophages and how they prepare them for assays. Specifically, I am interested in knowing if there is an intrinsic difference between non-adherent...

13 March 2023 4,955 5 View

Noise in ADP Glo assay?

I am using the Promega ADP Glo assay to look at inhibitors. I am getting a very large amount of noise in the assay. The noise is so bad that I cant reliably measure compound IC50s. I wonder if...

06 March 2019 1,281 2 View

How do Arrhenius plots change in the presence of enzyme activators or inhibitors?

Does the slope chg, or some other parameter?

02 November 2017 3,377 5 View

How does enhancement of fluorescence at the excitation wavelength occur?

I am looking at an NBD labeled lipid. I see a large enhancement of the excitation intensity but very little in the emission intensity upon addition of protein. I was wondering how this can occur...

02 October 2017 5,379 3 View