Dear Friends,

I am going to analyze the mcrA gene dataset. The trimming, truncating, chimera removing steps have been already done. Now, I need a database to do that. Please help me how can I find a database and how make it compatible with Qiime2? Or please tell me how can I make by myself? My major purpose is the taxonomy affiliation/ assignment.

* I found one but I got an error with some classifiers except Blast+. Its output showed only a few taxa, not more. I think the results were unreliable. Therefore, I need your help.

Article Taxonomic database and cut-off value for processing mcrA gen...