Is there a protocol to sort proximal tubule cells after Cre loxp recombination and to sort the rocombined vs the WT cells to then perform qPCR to approve that the cre lox recombination actually worked in deleting the floxed gene?
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..
26 February 2021 3,533 1 View
I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...
09 February 2021 375 2 View
I am using methyl violet 10B and reactive orange 16 (RO16) dyes for my Ph.D. research. I'm trying to find the molecular size for both of these dyes. It is possible for me to find these dyes'...
01 February 2021 3,333 4 View
I am performing pushover analysis in Etabs of a steel braced frame but oI do not know what value should I use for target displacement. The height of the building is 20m. I want to know what value...
29 January 2021 2,190 2 View
I want to coat the pellet because without electrical contact (may be due to the high resistance of the pellet), the V-I plot is not coming in the ZEM-3 instrument. I want to measure electrical...
24 January 2021 836 2 View
I have a dataset with an unusual configuration and was hoping for guidance on choosing a method to test for changes in mean. For context, this is operational data tracking vehicle arrivals at 10...
20 January 2021 4,429 4 View
Currently we start a chemical etching project. This project involved etching a polished titanium grade 5 surface part. We facing a problem where by our etch resist coating peel off during etching...
15 January 2021 1,592 3 View
13 January 2021 2,487 1 View
The new, more contagious variant of the virus that causes COVID-19 which initially detected in the U.K. and has now been spotted in many of the country. While some have referred to this as a new...
11 January 2021 8,810 5 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...
22 February 2021 2,125 3 View
22 February 2021 2,588 2 View
Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...
16 February 2021 9,050 5 View
To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...
16 February 2021 4,501 3 View
I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...
15 February 2021 8,034 7 View
Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...
14 February 2021 1,160 7 View
Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...
14 February 2021 2,039 4 View
I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...
14 February 2021 3,971 1 View