Is there a protocol to sort proximal tubule cells after Cre loxp recombination and to sort the rocombined vs the WT cells to then perform qPCR to approve that the cre lox recombination actually worked in deleting the floxed gene?
What are the most effective remote sensing techniques and algorithms for detecting and analyzing land use and land cover (LULC) changes in rapidly urbanizing areas, and how do their accuracies and...
11 July 2024 6,695 1 View
My attempt to promote listening was "Using Short-stories". It works fairly but there could be better options. Role of Short Stories in Developing English Listening Skill
09 July 2024 9,078 4 View
I have tried to resolve this issue a little bit, but eager to get your insights and thoughts to make my efforts better. Using Homophones and Homographs in ESL Classrooms
04 July 2024 6,742 3 View
The contribution of "Distance Education" is immense, but still needs a lot of reforms. Universities generally adopt one another ideas and ignore the ideas of world wide scholars, distance...
04 July 2024 2,545 6 View
"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...
01 July 2024 8,406 5 View
In educational institutions where English is taught as a second language, only 25% of listening happens in English but rather 75% of listening takes place in other language like Hindi/Urdu/Punjabi...
28 June 2024 6,353 4 View
Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...
25 June 2024 5,799 0 View
There is research into the models individually, but not one seeking to find an overall best one suited for any condition.
23 June 2024 5,641 3 View
I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...
12 June 2024 4,027 1 View
Hello everyone, My question for sure is silly, but I have been arguing with my labmate about this. If our plasmid concentration is 0.776 µg/µL and he wants to make a final concentration for...
10 June 2024 9,942 5 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
22 July 2024 7,811 4 View
I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...
16 July 2024 7,475 1 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
05 July 2024 4,070 3 View
I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...
26 June 2024 3,735 3 View
Dear researchers. I have extracted data from graphs for meta analysis. I am stuck to find the SD for the obtained data. Anyone who can help me to sort out this problem with formula? Thank...
23 June 2024 2,992 2 View
Lox p was integrated at correct location, but unable to express cre recombinase to delete gene. Performed at different log hour and different Galactose concentration. Even though no gene deletion...
17 June 2024 1,069 3 View
I have a longitudinal dataset where I am looking to examine whether individuals change their category membership of a variable (with four categories) over four time points (baseline, 1y, 2y, 3y)....
16 June 2024 9,770 10 View
Porn to men is not a big deal. They honestly can’t see how watching a sexy film can be any sort of reflection on their love for their girlfriend. Most don’t understand why she takes offence...
16 June 2024 9,513 0 View
The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...
13 June 2024 2,590 4 View