Protocol of Enzymatic activity?

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How can I calculate accurate brick-joint interface stiffness for masonry in DIANA FEA?

Greetings everyone. To define the interface between mortar and brick during the modeling of masonry, I need normal and shear stiffness (Kn and Kt). I have tried some relationships found in the...

23 June 2024 6,015 0 View

How can I imagine the structure of the intra-band when we mention dynamics of the electron in the intra-band and it's transition ?

The non-linear response of the electron in the driving laser field, we always mention in solids, inter-band polarization, and coherent intra-band dynamics of the electron. I want to deepen my...

17 April 2024 3,406 1 View

What is the impact of using shellfish and limestone on pultry production performances?

Effet of shellfish and limestone on growth performance, carcass characteristics, biochemical characteristics of blood, and physico-chemical and techno-functional of broiler chicken meat. Effet of...

20 March 2024 5,388 0 View

Are there any predefined functions in Matlab for approximating a function or parameter using TS fuzzy logic?

21 February 2024 3,621 4 View

How study the access of lexical-semantic system in patients with cranial injury with IRMF & EEG?

18 February 2024 1,162 1 View

How to correctly integrate a Wind Turbine with DFIG in a grid using PSAT?

I am receiving this warning frequently: Warning: DFIG at bus : iqr is under its min limit. Doubly fed induction generators cannot be properly initialized.

29 August 2023 1,923 0 View

In a co-culture, is there any risk of mixing two different media RPMI and DMEM together?

Each of cell lines grows in a different medium. One in DMEM and the other in RPMI. Thanks

24 November 2022 4,615 3 View

I have only Two (2) constructs in PLS, how to do HTMT?

Hello dear researchers, I have only Two (2) 2nd order constructs in my model, the first one is Reflective-Formative (Independent) and the second one is reflective-reflective(dependent). I would...

15 November 2022 9,749 0 View

How to calculate multiplicity of infection?

I am going to infect caco-2 cells next week with an E. coli strain. The protocols I have have the following steps: seed 4x10^5 caco-2 cells overnight infect with bacteria with a multiplicity of...

16 June 2022 6,186 2 View

Dna extraction from soil?

I would bé gratful if you could send me a protocol to extracted metagenomic Dna from soil. The aime of work is shotgun metagenomics sequencing,so i'm looking for a good yield ans concentration. I...

09 June 2022 496 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Engelbert Buxbaum

Substrates that produce fluorescent products are probably easiest to use: fluorescein diacetate produces a soluble product, ELF-97 acetate a precipitate.

Pablo Pérez-García

Just induce the expression, prepare a cell extract and test it against para-nitrophenyl esters or 4-Methylumbelliferone esters. Use a negative control with an uninduced extract.