Somebody would have a protocol of neurosphere transfection to share ?
Thanks
Hello
Look at links
Good Luck
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083596
http://cancerres.aacrjournals.org/content/canres/suppl/2012/06/26/0008-5472.CAN-11-3490.DC1/meth_125K.pdf
hi
best transfection method for primary or stem cells is lentiviral particles ( transfectant rate and stability of recombinant clones is more than other methods).
but you can used 4D-Nucleofector™ system from lonza company .
http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_AppNotesTossSheets_Optimization_of_mouse_neural_stem_progenitor_cell_NSC_transfection_with_the_4D-Nucleofector_system.pdf
Thank you all for your answers !
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