Going from pyruvate to ethanol is a two-step process. In the first step, a carboxyl group is removed from pyruvate and released in as carbon dioxide, producing a two-carbon molecule called acetaldehyde. Alcohol fermentation by yeast produces the ethanol found in alcoholic drinks like beer and wine. For protocol follow these links and read the attached document, please https://www.ajol.info/index.php/ajb/article/viewFile/135792/125289Article Alcoholic Fermentation with Flocculant Saccharomyces cerevis...
Dear Nikunj Patel
The seed culture contained 10 g/l of glucose, 0.45 g/l of NH4Cl and 1 g/l of yeast extract. The medium was autoclaved at 121 °C and 15 psig for 20 min. The sterilized medium was inoculated with 5% of pure seed culture of the microorganism and then the culture was cultivated in an incubator-shaker at 30 °C for 24 h.
After making seed culture, at optimum conditions the medium by optimum concentration of substrate, 3 g/l of yeast extract and 5 g/l of NH4Cl must be prepared. The pH value was set at 5.2 provided by buffer solution of potassium hydrogen phthalate (0.1 M) and sodium hydroxide (0.1 M). Fermentation has carried out at 32 °C by inoculation of seed culture.
Ethanol fermentation can be performed in conventional process or via batch and continuous fermentation in membrane bioreactor.
If you need additional information can look for my paper entitles:
- Composite MWCNT.PDMS Membrane Bioreactor for Enhanced Bioethanol Production from Broomcorn Seeds. International Journal of Engineering.Transactions A: Basic, 31 (2018) 516-523.
I tried to give you the most complete answer to your request.
Best regards
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