We plan to isolate mitochondria, OMM, Matrix, Golgi, ER, lysosome and nucleus using differential centrifugation approach. Can anybody share with us the protocol for the aforementioned along with the buffer to isolate
please specify the organism and cell type (if applicable) you want to do a subcellular fractionation with. This information would be useful to guide you.
thanks for your reply. I have no practical experience with the cell types you mention so I can't give you a good answer how to do a subcellular fractionation.