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04 May 2018 5,196 3 View

Can anybody who is familiar with JC-10 (Mitochondria membrane potential dye) staining?

09 October 2016 2,865 1 View

I want to try to use HeLa cell and lipofectamine 2000 to do transfection?

I am trying to optimize the transfection by using lipofectamine 2000 for 2000 ng of plasmid DNA/ well with different amounts of lipofectamine 2000 (5ul, 10ul) in the 6-well plate. There is a...

03 April 2016 6,490 6 View

I am interested in autophagosome study in cell line like HEK 293?

plese provide the protocol how we can go through transfection of the cell using the GFP tag etc, positive control of the expression system to check the expression of the channel?

01 February 2016 8,529 4 View

Can someone explain how to calculate the cell capacitance?

I am recording the sodium currents in whole cell patch clamp methods in DRG neurons (20 micrometer) from the Axopatch-200B and getting capacitance 1.24 pF in the dial. Should the capacitance be...

02 March 2014 9,962 9 View

Narmalized Current

I am affraod to ask if any one suggest me How we can normalized the sodium current recorded from the whole cell configurations and calculation of tau value. Iam normalizing with positive value of...

02 March 2010 3,343 0 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Can you apply graph theoratic functional connectivity analysis to study cognitive processes under external stimuli?

Can functional connectivity analysis using Graph Theory (Hub-ness, clustering coefficient, etc) be applied to study cognitive processes? I felt most of the analysis to study the brain after...

25 January 2021 4,575 3 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

What are some recent publications showing the predictor power of resting state brain acitivity for tasks done outside the MRI scanner?

Looking for recent articles (2018-2020) that showed resting state brain activity can predict cognitive task performance (e.g., attentional and executive functioning tasks) done in the lab AFTER...

09 January 2021 2,310 2 View

What are the essential elementts of a Nature Neuroscience Paper?

What would land a neuroscience paper in Nature Neuroscience? What is the minimum a paper should have to pass the editorial scrutiny in Nature Neuroscience or the journals around the same cadre?...

01 January 2021 1,510 2 View