I am affraod to ask if any one suggest me How we can normalized the sodium current recorded from the whole cell configurations and calculation of tau value. Iam normalizing with positive value of current show it going to more than -1.
Please provide name of the the dealers / supplers for hERG (human ether-à-go-go-related gene) transfected either CHO or HEK-293 cells. I got some quotaions seems to be very expensive. we need for...
04 May 2018 5,291 3 View
I am using JC 10 stained for mitochondrial membrane potential. if anybody is using can you please suggest me the protocol for staining with JC-10 dye. thanks in advance
09 October 2016 3,009 1 View
I am trying to optimize the transfection by using lipofectamine 2000 for 2000 ng of plasmid DNA/ well with different amounts of lipofectamine 2000 (5ul, 10ul) in the 6-well plate. There is a...
03 April 2016 6,688 6 View
plese provide the protocol how we can go through transfection of the cell using the GFP tag etc, positive control of the expression system to check the expression of the channel?
01 February 2016 8,638 4 View
I am recording the sodium currents in whole cell patch clamp methods in DRG neurons (20 micrometer) from the Axopatch-200B and getting capacitance 1.24 pF in the dial. Should the capacitance be...
02 March 2014 10,126 9 View
I want to study the effect of long term treatment (14 days and 28 days) of a compound in olfactory bulbectomized (OBx) rats on locomotor activity (open field), aggressive behavior and sprouting...
03 April 2011 9,875 2 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...
17 July 2024 1,225 0 View
The main differences
14 July 2024 3,158 4 View
I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...
23 June 2024 4,083 1 View
We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...
06 June 2024 2,234 1 View
Does anyone use or know of any internationally recognised and reliable publishing outlet (i.e. Journals) that are specialised for submission of "futuristic" articles which are based on the...
28 May 2024 2,946 2 View
Dear all, I need your expertise on this matter. I have an article under review and I got some comments that I need your help with: In my research, I investigated the FRN (200_300ms post_feedback...
23 May 2024 4,953 0 View
I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...
23 May 2024 7,776 2 View
I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?
12 May 2024 4,614 3 View
I am using a biphasic electrical stimulation on neural networks to attempt to induce electrical kindling for epilepsy on neural networks. In addition, I will be attempting to modulate said...
09 May 2024 7,167 1 View