Protocol for micro chromatin Immunoprecipitation?

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What analytical equations are used to calculate nuclear gradients in dft?

I'm looking for an explanation of how the calculation of nuclear gradients works in DFT in the context of geometric optimization. Mostly, I want to know what equations are used to calculate...

03 November 2023 8,144 1 View

How to remove dirt from diamonds for AFM study?

Hello everybody. I'm trying to study HPHT synthetic diamond plates with low (first nm roughness). When i taking 100×100 μm photo by AFM, you can see a lot of flakes/dirt on them. The question is...

25 October 2023 7,952 4 View

How to freeze atomic positions and set atomic charges when optimizing geometry in pybel?

I'm trying to do something like docking, but for crystals, in the task of transcribing XANES spectra, estimating the random positions of inserted atoms into a fixed crystal structure using a graph...

23 October 2023 3,850 0 View

What type of filter should be used when printing with titanium powder in an SLM printer?

Dear Colleagues! I would like to know your advice about printing with titanium powder in an SLM printer. Will a HEPA filter be enough to filter the gas from residual printing products when working...

20 August 2023 9,837 1 View

How can I automatically get charges of each carbon and nitrogen atom in skeleton of molecule after gaussian calculation?

Hi, I have a list of output files after a Gaussian calculation, and I want to get the charges of carbon and nitrogen atoms in the constant skeleton of these molecules to use them later as QSAR...

17 July 2023 6,438 1 View

What is the place of PLTs in the actual practice of foundation design in the US? What US documents regulate calculation methods?

Hello! I am doing a review on Plate load test in global practice. I have started by looking at foundation design practices in general and I have questions when looking at the US code system and...

25 June 2023 3,166 2 View

Litterature on China-ASEAN sustainable cooperation?

Dear all, cuurntly im a writing a graduation paper on the topic of cooperation between China and ASEAN in terms of sustainable development. I've already found quite a few official reports and...

02 March 2023 7,435 0 View

Tell me what the arrows in the algorithm mean ?

I would like to know the translation, what does the word mean "zoom" (translation needed word. I will be very grateful for the answer!

19 February 2023 1,569 0 View

Google Scholar Preprint Bug - any way to avoid?

The bug when published paper did not appear in Google Scholar after posting preprints at BioRxiv and ArXiv has been described in 2014:...

29 November 2022 6,857 0 View

What post-hoc is appropiate for repeated measures ANOVA?

Dear colleagues! I have two groups of animals which I compare using 8 day long behavioral paradigm. For each day I have two data points (day and night). Thus I have one independent factor (animal...

04 August 2021 4,490 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View