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I would like to do a transformation with E. coli ATCC 25922. Would like to simply add a plasmid for amp. resistance. I need to use ATCC 25922 for my research question. Does anyone have a protocol...
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I have plasma from knockout mice treated with vehicle or raloxifene/tamoxifen. Looking for a small acute phase protein (~12 kDa). I first did a 1:1 dilution of sample to laemmli buffer, but the...
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