QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

What purity grade of PEG-300 and NMP should be used to dissolve drug for in vivo use?

Hi. I need to dissolve a drug to be given to mice by oral gavage. Based on a paper I found, the drug should be dissolved in 10% (vol/vol) NMP/40% (vol/vol) PEG-300/50% (vol/vol) water. Looking...

16 August 2020 8,685 2 View

Question for psychtoolbox users. Divided the screen?

*Question for psychtoolbox users* I using one screen. The function "flip" flips all screen. Can i divided the screen to two areas of "flip"? I have two stimuli with different time display flipping...

10 August 2020 4,828 3 View

Which mouse strain is best suited for HCT116 xenograft experiments?

I would like to perform a xenograft experiment with HCT116 cells on immune compromised mice. I was wondering which mouse strain would be best suited for such an experiment?

05 August 2020 9,437 3 View

What restrictions or guidelines do you know pertaining to Wind Turbine Noise in your country or others?

I'm looking for any kind of legislation, zoning, best practices, etc. either required or recommended by the state, the EU/other federation or local government.

17 January 2020 4,987 2 View

How to conduct moderation model in a mixed design?

I have a mixed model in which I have: - within independent variable with three levels (emotion regulation strategies) - between independent variable, continuous (fear of height questionnaire...

24 May 2018 4,107 2 View

Can any one give me a protocol for beta gal ONPG assay for AML12 transfected cells?

I have a protocol which we used a few years ago with 3T3 cells. With the AML12 cells I don't get any color. Any suggestions? Thank you.

30 October 2017 1,749 1 View

How to treat scalar transport equation with AUSM type scheme?

06 August 2017 9,717 9 View

Why is it common to treat multiphase flow as incompressible?

Hi, It is known that the speed of sound in the mixture region between two phases is lower than the speed of sound of each phase. Therefore, is it still justified to use incompressible formulation...

22 May 2017 5,891 6 View

Anyone familiar with high-order positivity preserving limiter?

26 April 2016 1,153 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View