I am trying to conduct a metaproteomic study for rhizosphere proteins. Please direct me to a protocol that allows for a good quantitative estimation and a clear MS result. thanks!
Hello Amit,
many thanks for sharing this interesting technical question with other RG members. As an inorganic chemist I'm absolutely no specialist in this field of research. Thus all I can do at the moment is suggest to you a potentially useful literature reference. I think that the following review article should be very valuable with respect to your question:
Soil Metaproteomics for the Study of the Relationships Between Microorganisms and Plants: A Review of Extraction Protocols and Ecological Insights
Article Soil Metaproteomics for the Study of the Relationships Betwe...
Fortunately this paper has been posted by the authors as public full ext on RG. Thus you can freely download it as pdf file and print it out.
Good luck with your research work and best wishes, Frank Edelmann
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