09 September 2013 4 2K Report

I am looking for a protocol for an efficient lysis of bacillus megaterium, to decrease the sonication time?

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Why does lysozyme affect Gram-positive bacteria in the exponential growth phase better than in the stationary phase?

If lysozyme has the ability to attack the binding between NAG and NAM, it should be able to do that during all growth phases. But some bacteria (Gram-positive) become resistant to lysozyme in the...

09 October 2013 7,949 5 View

When using phusion polymerase, should I use an annealing temp, which around the calculated tm of the primers pair?

for the PCR I use the Phusion polymerse from NEB rather than Pfu or Taq... Using Pfu and Taq the annealing Temp is 5- 10 grade lower than the calculated Tm, but what about the Phusion polymerse,...

06 July 2013 8,322 6 View

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