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Questions related from Mohammed Milhim
If lysozyme has the ability to attack the binding between NAG and NAM, it should be able to do that during all growth phases. But some bacteria (Gram-positive) become resistant to lysozyme in the...
10 October 2013 7,921 5 View
I am looking for a protocol for an efficient lysis of bacillus megaterium, to decrease the sonication time?
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for the PCR I use the Phusion polymerse from NEB rather than Pfu or Taq... Using Pfu and Taq the annealing Temp is 5- 10 grade lower than the calculated Tm, but what about the Phusion polymerse,...
07 July 2013 8,281 6 View