Proteus Mirabilis Subcultures?

More Harpreet Sangeh's questions See All

How to do wavelet transform of EXAFS data?

I want to do wavelet transform of some compounds for their comparative study? Kindly help me in this regard and provide with proper procedure.

31 July 2024 4,170 0 View

How to analyse EXAFS Wavelet transform spectra?

I am analysing exafs spectra of some heavy metal compounds. I tried exafs fititng for compounds in artemis but not getting final parameters in permissible range and not even proper fit for some...

07 July 2024 2,812 4 View

Why are my cells dying during puromycin selection after lentiviral transduction?

I have knocked down my gene of interest by lentiviral transduction. I revived the vials and continue growing cells for 72 h without any selection. After confluency, I passaged the cells later 24...

28 February 2024 4,032 1 View

How to separate the high molecular weight proteins (>300kDa) in SDS-PAGE?

Hello everyone! In a SDS-PAGE, what is the more effective way to separate high molecular weight protein st (>300 kda) and get a good resolution at the end? I am using Tris-acetate 4-8% gel and...

18 June 2023 7,104 2 View

Contemporary Psychodynamic Authors who are Existential?

Hi everyone, can anyone suggest any authors that bring psychoanalysis and existentialism together, and if possible, could you recommend/suggest any texts? Thanks in advance.

26 July 2022 8,392 12 View

Which is the Best method for cell type annotation for single cell characterization ?

Hi Everyone, I have query regarding cell type annotation for single cell characterisation. Whether automated annotation (based on identified clusters) methods or based on known marker genes...

29 June 2022 3,879 6 View

Is the Electric Vehicle (EV) the solution of our mobility in the future?

Electric vehicles (EVs) are now widely regarded as mature and promising for the future of mobility. As the markets expand, the next battery technologies are being marketed as a miraculous answer...

08 May 2022 642 4 View

Could any one suggest at which standard, speed and load we can observe tensile strength for a cardboard made from domestic waste?

08 December 2021 4,809 0 View

Plasmid for CRISPR in plants?

Hi all. I am looking for a plasmid to do a gene knockout in Arabidopsis thaliana. I would like to have a fluorescence gene (RFP/GFP), antibiotic resistance (kan/amp/sm), Cas9, At promoter and also...

23 June 2021 4,740 1 View

Active area in perovskite solar cell fabrication?

Hello, I am a Ph.D. student and starting work on Perovskite solar cells. I want to know what active areas of solar cells should I try to get the best results?

22 April 2021 6,385 7 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

12 July 2024 7,027 0 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

Can someone provide any tips for cell quantification on Fiji, ImageJ?

Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...

05 July 2024 4,197 1 View

Cloning restrction digestion needed before sequencing?

Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...

04 July 2024 7,977 2 View

Why I am unable to get the expected insert at the cloning site?

I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...

02 July 2024 5,448 2 View