Hello, I have a membrane protein reconstituted in amphipol. When checked with 200k, I found that protein couldn't enter into the center of squares, but only the edge holes of the squares. And as if samples entered into holes, it could dispense in the whole hole. In this case, how can I optimize grid condition? A higher glowing current, a longer waiting time, or something? or if I just make grids in detergent not in amphipol, can it improve? Thank you so much.
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