Xinyu Teng

6 Questions 1 Answers 0 Followers

Questions related from Xinyu Teng

Does anybody have some ideas about cryoEM data processing for small membrane protein?

Hi, here I am run data processing on a small memvrane protein(

09 January 2023 4,447 0 View

Does anyone know how to process data of small membrane protein with only TM domain?

Hello, now I am processing a 300kv cryoEM data by relion for a small membrane protein(~100kd), which only has transmembrane domain. However, when undergoing 3d classification, I found protein...

04 January 2023 1,767 1 View

Does anyone meet the problem that nanodisc size is not homogenoous?

Hello, when I re-consituted my protein into nanodisc and checked under cryoEM, I found nanodisc diameters were not so honogeneous. Has anyone ever met this problem? does it mean the ratio of MSP...

08 December 2022 7,578 1 View

Does anybody know how to calculate the best ratio of preotein, MSP and lipid in nanodisc?

When we reconstitute one protein into nanodisc, how we get the best ratio for protien, MSP and lipid? Is there any fomula or cenrtain ratio that we can reference?

15 August 2022 3,926 1 View

Protein can't enter into hole while making grid?

Hello, I have a membrane protein reconstituted in amphipol. When checked with 200k, I found that protein couldn't enter into the center of squares, but only the edge holes of the squares. And as...

25 December 2021 2,303 0 View

Does anyone know the meaning of soft edge in mask creation by relion?

Hello, everyone. I want to try multi-body refinement with relion acccoding to this protocolMulti-body Refinement of Images in. But I want to understand the meaning of " the final masks do not have...

01 January 1970 8,190 0 View