does somebody knows a good protocol that can extract both DNA and/or protamine and histones from mouse sperm?
Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...
29 July 2024 4,630 0 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Saludos a todos. Recientemente he tenido inconvenientes con el programa de ajuste de circuitos Eisanalyser ya no abre para Windows 11 y 10, hace unas semanas funcionaba bien y dé repente dejó de...
21 April 2024 7,798 1 View
Espera-se encontrar artigos que abordem meios de inserir as tecnologias digitais nas práticas pedagógicas da Educação Infantil de forma saudável, controlada e segura. Trabalhos bibliográficos ou...
15 April 2024 6,789 0 View
Hello, I've been scouring the internet for a scientific article that models Sustainable Development Goal 8 (Decent Work and Economic Growth) using Ordinary Differential Equations (ODE), but I...
30 March 2024 8,477 3 View
Nos enfocamos en como diseñar estrategias participativas para involucrar a los mayores, y lo más importante es que respete su autonomía y dignidad. Buscamos entender como incluirlos en la toma de...
10 March 2024 2,659 3 View
Hello, I have available TEM copper grids coated with Formvar but not carbon. I would like to visualize Viruses in suspension from a concentrated sample. Would the lack of carbon coating affect my...
06 March 2024 1,219 0 View
Hello, I have available a Solarus II Plasma Cleaner. Would plasma cleaning glow discharge formvar coated TEM copper grids? I want to visualize viruses in suspension. Thank you, Paula
06 March 2024 7,453 0 View
Hello! Recently, I ordered an FOLH1 ORF, which came inserted into the pUC-57 mini vector. Additionally, I requested BamHI and XbaI restriction sites to be included at each end of the ORF. When I...
25 February 2024 7,328 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View