What is the most general protocol for UV-light induction of prophages? What light intensity and time is generally used for such treatment?
Dear Tatiana:
here's a valuable pdf article about your question ...
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. The wild type of this virus has a temperate life cycle that allows it to either reside within the genome of its host through lysogeny or enter into a lytic phase, during which it kills and lyses the cell to produce offspring. Lambda strains, mutated at specific sites, are unable to lysogenize cells; instead, they grow and enter the lytic cycle after superinfecting an already lysogenized cell ...
Multiplicity reactivation (MR) is the process by which multiple viral genomes, each containing inactivating genome damage, interact within an infected cell to form a viable viral genome. MR was originally discovered with phage T4, but was subsequently found in phage λ (as well as in numerous other bacterial and mammalian viruses[14]). MR of phage λ inactivated by UV light depends on the recombination function of either the host or of the infecting phage.[15] Absence of both recombination systems leads to a loss of MR.
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Survival of UV-irradiated phage λ is increased when the E. coli host is lysogenic for an homologous prophage, a phenomenon termed prophage reactivation.[16] Prophage reactivation in phage λ appears to occur by a recombinational repair process similar to that of MR.
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