How to get rid of the free GFP signal in an Atg8-GFP tagged yeast saccharomyces cerevisiae?

I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...

15 November 2020 8,556 3 View

Hi all! I need to activate CD8 T cells extracted from mice spleen in vitro, which option is the best? Concanavalin A? LPS? anti-CD3 and anti-CD8?

in vitro mice CD8 cell activation. These cells are extracted from B6 mice spleen by magnetic separation.

01 November 2020 3,276 2 View

Hi all! What vehicle do you recommend for IP injection in mice for a DMSO soluble drug?

I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....

17 September 2020 9,107 3 View

Why is Quantum-dot Cellular Automaton (QCA) technology fail to flourish? Specially, Semiconductor and Magnetic QCA for electronic applications?

In 1993, Lent et al. introduced one of the most promising and emerging computational paradigm known as Quantum-dot Cellular Automaton (QCA) computing. Now, it is almost 30 years have been passed,...

06 May 2020 4,955 7 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

Which are the best journals which accept newcomer students for writing reviews on topics related to biomedical engineering ?

I am looking to communicate with a personnel who deals with beginner-level review articles, and want to learn how to write good reviews and the rules and regulations to follow.

06 March 2020 6,068 3 View

In which reason EDTA used in injectables containing proton pomp inhibitors?

We know that EDTA is used by different reasons (chelating agent, support for antimicrobial effectiveness e.g) in pharmaceuticals. Is there any specific reason to prefer EDTA in injectables...

01 December 2019 5,166 3 View

How to get mean age of fluid for a closed system using FLUENT?

Hi everyone, I am working on simulation of stirred tank reactor operating at batch mode. So, there is no fluid inlet-outlet in the system. Can I calculate the local mean age of fluid by using...

18 November 2019 1,142 3 View

Grain model software?

Could You please recommend me a CFD software for surface reaction (like ZnO+H2S=ZnS+H2O) simulation with ability to calculate shell diffusion resistance by grain models like (non-overlapping grain...

14 July 2019 6,285 2 View

How is the length of any scientific document (report, thesis, essay, conference paper, journal article) calculated ?

I wish to know what aspects of these technical documents are included and excluded while making a word count. Specifically, say, a figure caption, references, tables, table footnotes, page...

24 June 2019 9,815 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View