Anti-Src and anti-pY418 Src antibodies for IF?

Hi everyone, Does anyone have a suggestion for anti-Src and anti-pY418 Src antibodies that work well in immunofluorescence on cultured neurons? Thanks a lot!

26 January 2021 866 1 View

Fluid flow simulation on electroplating tank. Are walls treatment important?

Hello everybody, I would like to perform a CFD simulation to visualize the fluid flow in an electroplating tank. This big tank (400 L) is mixed with several eductor nozzles. I would like to know...

13 August 2020 7,426 3 View

What is the impact of philanthropically-minded entrepreneurs in the institutionalization of social innovation?

Curious about the idea, that philanthropically-minded entrepreneurs can help to diffuse/institutionalize social innovation due to their belonging to at least two matured fields of business and...

12 August 2020 4,117 3 View

Are there any tools to extract data from facebook pages (posts, comments, etc.)?

Since it is not possible to work with Netvizz anymore I was hoping to find another tool which works similarly.

26 March 2020 959 3 View

Is there any reason why I can not order an oligo and use it directly as an insert for SLiCE cloning?

I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...

18 March 2020 2,996 3 View

Sars-cov-2 (Covid-19): qPCR or serological antibody testing - is there information?

So far I only read about PCR testings to confirm a patients infection. Is somebody aware of other diagnostical testing options? E.g. for EBV (Ebstein Barr Virus) exists a serology testing method...

12 March 2020 3,965 6 View

Which problems in fire fighting could be addressed with mobile robots? For which industrial scenarios would such a fire fighting robot be helpful?

I am looking for narrowing down the topic for my master theses. Real industrial problems to be solved with innovative technologies are the most interesting topics.

30 April 2019 5,506 6 View

What are some good examples (case studies) for using open badges in higher education?

I am writing a post about open badges, and researching some examples.

02 April 2019 4,484 2 View

Can anyone suggest a software for designing primers and internal oligos on bisulfite converted DNA, instead of Beacon Designer?

07 June 2018 6,063 2 View

How to install Neo (Python package for working with electrophysiology) for MAC?

I am wondering if it is possible to install Neo (Python package for working with electrophysiology) http://neo.readthedocs.io/en/0.5.0/ on a Mac. If yes, how to do it?

21 February 2018 2,857 1 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View