The crosslink of a purified protein to NHS-activated magnetic beads works absolutely fine. But now I want to crosslink human skin tissue lysate to the beads and this does not work at all. The buffer does not contain amines (pure PBS). I personally assume that the high lipid content of the tissue lysate interferes with the esterification. Therefore, I need a quick and easy protocol for lipid extraction. I want to get rid of the lipids and purify the proteins from the skin tissue lysate. I tried two consecutive rounds of precipitation with acetone, but it didn't solve the problem. Any suggestions of how to get rid of lipids from skin tissue lysate?

Similar questions and discussions