I have been constructing some promoter fusions using the lux operon as a reporter in a miniTn7 transposon, which is inserted in a neutral region in the genome.
Everything is fine until I conjugate the transposon into my strain (A. baumannii). When I re-streak colonies into selective plates that should let grow only my strain with the minTn7 inserted, some of them show some light while some others don't.
Why should this be?
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Use of Transcriptional Fusions To Monitor Gene Expression: a ...
jb.asm.org/content/176/7/2128.full.pdf
by AJ Forsberg - 1994 - Cited by 54 - Related articlesGene fusions are frequently used to facilitate studies of gene expression and promoter activity. We have found that ... problem is frequently circumvented by generating transcrip- ..... leuP-lux (leuPpromoter fragment cloned into pCH257).
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