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Questions related from Sharmila Srinivasan
I am trying out a new antibody (tubulin T6074 from sigma). I am repeatedly getting non specific bands all over the membrane and having difficulty in identifying my band of interest. I used 5% milk...
26 December 2017 8,782 0 View
Is it enough to have only have loading control for western blots? or it is necessary to include positive,negative controls as well. The concerned antibodies have been used already by many people...
11 April 2016 4,771 3 View
Cx43 is usually present on the plasma membrane of HaCaT cells. Immunofluorescence staining for Cx43 showed the staining in cytoplasm, which is wierd. I tried running western blots with total...
04 April 2016 6,249 1 View
My encapsulated compound is essential oil. When I run it in UV I get lots of peaks which look like noise. They look the same even when diluted. In similar papers they report a particular...
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