Sharmila Srinivasan

5 Questions 1 Answers 0 Followers

Questions related from Sharmila Srinivasan

Problems with non specific bands in alpha tubulin (T6074 SIGMA)???

I am trying out a new antibody (tubulin T6074 from sigma). I am repeatedly getting non specific bands all over the membrane and having difficulty in identifying my band of interest. I used 5% milk...

26 December 2017 8,834 0 View

Controls in western blotting?

Is it enough to have only have loading control for western blots? or it is necessary to include positive,negative controls as well. The concerned antibodies have been used already by many people...

11 April 2016 4,914 3 View

Are there any reasons why HaCaT show the presence of Cx43 in the cytoplasm?

Cx43 is usually present on the plasma membrane of HaCaT cells. Immunofluorescence staining for Cx43 showed the staining in cytoplasm, which is wierd. I tried running western blots with total...

04 April 2016 6,389 1 View

Are there any alternative methods for in vitro release or how to find a particular wavelength for an oil in a capsule?

My encapsulated compound is essential oil. When I run it in UV I get lots of peaks which look like noise. They look the same even when diluted. In similar papers they report a particular...

19 March 2014 10,026 1 View

Can oils be lyophilised?

I want to take FTIR for turmeric oil. At my place only powder samples are analysed and the oil is not freezing. Could anyone help me with this problem?

09 January 2014 3,635 1 View