Problems with insitu hybridization?

01 December 2023 0 6K Report

I m trying with the Insitu hybridisation for Cd31 in P0 mice in cryosections of 10um.

I don’t see any signal .

I tried with digoxigenin labels. I used riboprobes. Can anyone suggesr me or good protocol or good riboprobes for Cd31 (not the commercial)?

Thank you

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