Problem with IMR-90 cell culture, why do they all die?

17 March 2021 1 8K Report

I'm trying to start a culture of IMR90 and they do not seem to stick to the bottom of the flask. I coated the flask with collagen to help adherence, I'm using this media: EMEM with 10% FBS. Please help.

Davood Zaeifi

Dear Joannie Lemaire

Consider the attached protocol.

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