Sending out a Bat Signal to anyone who has successfully extracted DNA Primnoidae- especially from Thouarella and Dasystenella: what protocol did you use and what 'tweaks' did you use to successfully get DNA from these genera please?
I have 8-15 yr old samples that have been preserved fairly well. I've used McFaddens CTAB and then some other adaptations thereof plus salting out etc. I've used up to 9 polyps as starting material. Any ideas here that worked for you that I could try please? Any help would be greatly appreciated.
I recommend the method described in this paper
Article DNA extraction from sea anemone (Cnidaria: Actiniaria) tissu...
I assume you've had a look at this one. They do say that they had "more success with fresher material".
Article A phylogenetic analysis of the Primnoidae (Anthozoa: Octocor...
Results so far: worst extracts with QIAGEN DNeasy, CTAB (McFadden, Coffroth protocols) worked well, best result: salting out with modifications (gel attached). As expected, preservation methods and sample age majorly influence the outcome.
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