What do u mean by unknown gene?

Could be little clear

Dear Faezeh Mr Purushotham is right.

what do you mean by unknown gene.

it must have name if you are calling it a gene. or its protein must be know

or atleast its location in the genome..

you must know something about it to call it a gene.

if any of it is know there are ways to design primers for it..

if you please be more specific and clear I guess we can help you.

thanks .

Dr. Vivek SIngh

Hi,u'r right

imean i want to amplify a lysozyme gene from kidney of Rutilus frissi kutum, that has not been sequence determined yet ,just know it existes in tissue from which RNA extracted.

now i want to designe primer for a lysozyme gene of a similar spicies(cyprinus carpio)thate probably proliferate my lysozyme gene.OK?As its sequence is determined.

is it trusty enough?do you suggest other ways?

sincerly.

Faezeh

Dear Faezeh

If you want to check the expression of this gene you may take a small fragment (most conserved) but if you are planning to get it sequenced full then you need to go for primer design from most related or close species lysozyme sequence. Most conserved region generally are good for primer designing.

hope this is useful.

best of luck for your work..

Dr. Vivek Singh

Dear Faezeh Moradi,

If your gene contains introns and if if u plan to do from RNA, do sequence alignment of closely related species select conserved sites of your protein and design primer this would be forward primer, for reverse u can use oligoT. For getting full length gene u can do 3' and 5' RACE

By using the nuclotide sequence of the gene u design the primers easily by using tools like generunner,oligoexplorer,oligo7... While designing primers make sure that the primers donot form hairpin loops,bulge loops and dimers...