Hello! I am a 1st year PhD student, and am attempting to isolate three different types of ganglion cells from Nematostella vectensis for some in vitro studies. I have been through a few different publications to see how others had done it, and I have a pretty good idea of how to obtain a general culture of different cells, but I am not sure how to select and isolate ganglion cells to try and make a stable line. If anyone has any advice on how to do this, I would greatly appreciate your feedback!
Dear Hannah Justin
These papers and link might be useful, have a look:
1. https://www.nature.com/articles/s41598-021-83549-7
2. https://www.frontiersin.org/articles/10.3389/fendo.2020.00063/full
3. https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-016-0282-1
4. https://pubmed.ncbi.nlm.nih.gov/22159579/
5. https://royalsocietypublishing.org/doi/10.1098/rstb.2015.0065
6. https://www.sciencedirect.com/science/article/pii/S0012160617302452
7. https://github.com/klarman-cell-observatory/RetinalGanglionCell-ONC
Best Regards
Dr. Fatemeh Khozaei
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