I am interested in studying the effects of diets with varying amounts of fatty acids in Danio rerio, namely with high amounts of individual fatty acids. I am not above preparing my own feed if necessary but my question is, what might the best methods be for preparing such a recipe? Rather, is adding a purified fatty acid to a feed (or into a recipe for such) the best route to take?
Perhaps I am making things too difficult, but I can't seem to find anything in the literature that discusses such feeds- I am only finding references which discuss using manufacturer-provided feed with varying levels of many fatty acids. Thank you in advance, for any helpful advice.