09 November 2018 3 2K Report

Hey everyone,

I'm working with an esterase at the moment and I want to preform a pnp-acfetate assay. I'm just curious as to how people here prepare the stock solution of PNP-acetate. Basically I'm dissolving it in various concentrations of DMSO and pH 7 phosphate buffer but having trouble getting it to fully dissolve. I'm aware that lower pH is important too and I generally keep the pH at 7. I also want to keep the concentration of DMSO in the actual assay lower than 5%. Could anyone give me any tips as to how to prepare the substrate for the assay?

Thanks everyone

Harry

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